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phosphorylated src family kinase y416  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc phosphorylated src family kinase y416
    Phosphorylated Src Family Kinase Y416, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+phosphorylated+src/Phospho-Src+Family+(Tyr416)+Rabbit+mAb/pm40739316-162-42-48
    Average 94 stars, based on 68 article reviews
    phosphorylated src family kinase y416 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Western Blot:

    Article Title: Clonal GZMK + CD8 + T cells are identified as a hallmark of the pathogenesis of cGVHD-induced bronchiolitis obliterans syndrome after allogeneic hematopoietic stem cell transplantation
    Article Snippet: .. Western blot analysis was performed as previously described using whole cell lysates with the following primary antibodies: rabbit anti-COL1A1 (1:1000, Abcam), rabbit anti-FN1 (1:1000, Abcam), rabbit anti-Granzyme K (1:1000, CST), rabbit anti-Src (1:000, CST), rabbit anti-phosphorylated-Src (1:000, CST) or mouse anti-GAPDH (1:1000, Abcam). .. The membranes were then washed with PBST and incubated with goat anti-mouse or anti-rabbit-HRP secondary antibody (1:5000, Santa Cruz Biotechnology).

    Article Title: Isthmin-1 (Ism1) modulates renal branching morphogenesis and mesenchyme condensation during early kidney development.
    Article Snippet: .. Antibodies used for Western blotting, Co-IP and Integrin signaling blocking include mouse anti-ACTB (1:5000, A5316, Sigma), rabbit anti-GAPDH (1:5000, 10494-1-AP, Proteintech), mouse anti-FLAG (1:2000, F1804, Sigma), rabbit anti-Ism1 (1:5000, Genescript), mouse anti-V5 (1:3000, R960-25, Invitrogen), rabbit antiphosphorylated FAK (1:1000, #3283, CST), rabbit anti-phosphorylated AKT (1:1000, #9271, CST), rabbit anti-phosphorylated ERK (1:1000, #4370, CST), rabbit anti-phosphorylated SRC (1:1000, #2101, CST), mouse anti-N-cadherin (1:2000, #610920, BD), Goat anti-Gdnf (1:2000, AF212, R&D), HRP-conjugatedmouse (1:10000, GEHealthcare, NA9310) and HRP-conjugated rabbit (1:10000, GE Healthcare, NA9340). .. Total RNAwas isolated from CMMM-1 cells or tissues by TRIzol reagent (RNAiso Plus, Takara Bio, #9109) according to the manufacturer’s protocol.

    Article Title: Isthmin-1 (Ism1) modulates renal branching morphogenesis and mesenchyme condensation during early kidney development
    Article Snippet: .. Antibodies used for Western blotting, Co-IP and Integrin signaling blocking include mouse anti-ACTB (1:5000, A5316, Sigma), rabbit anti-GAPDH (1:5000, 10494-1-AP, Proteintech), mouse anti-FLAG (1:2000, F1804, Sigma), rabbit anti-Ism1 (1:5000, Genescript), mouse anti-V5 (1:3000, R960-25, Invitrogen), rabbit anti-phosphorylated FAK (1:1000, #3283, CST), rabbit anti-phosphorylated AKT (1:1000, #9271, CST), rabbit anti-phosphorylated ERK (1:1000, #4370, CST), rabbit anti-phosphorylated SRC (1:1000, #2101, CST), mouse anti-N-cadherin (1:2000, #610920, BD), Goat anti-Gdnf (1:2000, AF212, R&D), HRP-conjugated mouse (1:10000, GE Healthcare, NA9310) and HRP-conjugated rabbit (1:10000, GE Healthcare, NA9340). .. Total RNA was isolated from CMMM-1 cells or tissues by TRIzol reagent (RNAiso Plus, Takara Bio, #9109) according to the manufacturer’s protocol.

    Co-Immunoprecipitation Assay:

    Article Title: Isthmin-1 (Ism1) modulates renal branching morphogenesis and mesenchyme condensation during early kidney development.
    Article Snippet: .. Antibodies used for Western blotting, Co-IP and Integrin signaling blocking include mouse anti-ACTB (1:5000, A5316, Sigma), rabbit anti-GAPDH (1:5000, 10494-1-AP, Proteintech), mouse anti-FLAG (1:2000, F1804, Sigma), rabbit anti-Ism1 (1:5000, Genescript), mouse anti-V5 (1:3000, R960-25, Invitrogen), rabbit antiphosphorylated FAK (1:1000, #3283, CST), rabbit anti-phosphorylated AKT (1:1000, #9271, CST), rabbit anti-phosphorylated ERK (1:1000, #4370, CST), rabbit anti-phosphorylated SRC (1:1000, #2101, CST), mouse anti-N-cadherin (1:2000, #610920, BD), Goat anti-Gdnf (1:2000, AF212, R&D), HRP-conjugatedmouse (1:10000, GEHealthcare, NA9310) and HRP-conjugated rabbit (1:10000, GE Healthcare, NA9340). .. Total RNAwas isolated from CMMM-1 cells or tissues by TRIzol reagent (RNAiso Plus, Takara Bio, #9109) according to the manufacturer’s protocol.

    Article Title: Isthmin-1 (Ism1) modulates renal branching morphogenesis and mesenchyme condensation during early kidney development
    Article Snippet: .. Antibodies used for Western blotting, Co-IP and Integrin signaling blocking include mouse anti-ACTB (1:5000, A5316, Sigma), rabbit anti-GAPDH (1:5000, 10494-1-AP, Proteintech), mouse anti-FLAG (1:2000, F1804, Sigma), rabbit anti-Ism1 (1:5000, Genescript), mouse anti-V5 (1:3000, R960-25, Invitrogen), rabbit anti-phosphorylated FAK (1:1000, #3283, CST), rabbit anti-phosphorylated AKT (1:1000, #9271, CST), rabbit anti-phosphorylated ERK (1:1000, #4370, CST), rabbit anti-phosphorylated SRC (1:1000, #2101, CST), mouse anti-N-cadherin (1:2000, #610920, BD), Goat anti-Gdnf (1:2000, AF212, R&D), HRP-conjugated mouse (1:10000, GE Healthcare, NA9310) and HRP-conjugated rabbit (1:10000, GE Healthcare, NA9340). .. Total RNA was isolated from CMMM-1 cells or tissues by TRIzol reagent (RNAiso Plus, Takara Bio, #9109) according to the manufacturer’s protocol.

    Blocking Assay:

    Article Title: Isthmin-1 (Ism1) modulates renal branching morphogenesis and mesenchyme condensation during early kidney development.
    Article Snippet: .. Antibodies used for Western blotting, Co-IP and Integrin signaling blocking include mouse anti-ACTB (1:5000, A5316, Sigma), rabbit anti-GAPDH (1:5000, 10494-1-AP, Proteintech), mouse anti-FLAG (1:2000, F1804, Sigma), rabbit anti-Ism1 (1:5000, Genescript), mouse anti-V5 (1:3000, R960-25, Invitrogen), rabbit antiphosphorylated FAK (1:1000, #3283, CST), rabbit anti-phosphorylated AKT (1:1000, #9271, CST), rabbit anti-phosphorylated ERK (1:1000, #4370, CST), rabbit anti-phosphorylated SRC (1:1000, #2101, CST), mouse anti-N-cadherin (1:2000, #610920, BD), Goat anti-Gdnf (1:2000, AF212, R&D), HRP-conjugatedmouse (1:10000, GEHealthcare, NA9310) and HRP-conjugated rabbit (1:10000, GE Healthcare, NA9340). .. Total RNAwas isolated from CMMM-1 cells or tissues by TRIzol reagent (RNAiso Plus, Takara Bio, #9109) according to the manufacturer’s protocol.

    Article Title: Pulsatility and high shear stress deteriorate barrier phenotype in brain microvascular endothelium
    Article Snippet: .. Primary antibodies were diluted in PBS-T supplemented with 10% starting block solution (Thermo Fisher Scientific): rabbit anti-Zona Occludens 1 (ZO-1, 1:1000, Life Technologies), mouse anti-Claudin-5 (1:2000, Life Technologies), rabbit anti-P-glycoprotein (1:1000, Abcam, Cambridge, MA, USA), rabbit anti-GLUT1 (1:1000, Abcam), rabbit anti-phosphorylated-ERK (1:2000, Cell Signaling, Beverly, MA, USA), rabbit anti-ERK (1:1000, Cell Signaling), rabbit anti-phosphorylated-Src (1:1000, Cell Signaling), rabbit anti-Src (1:1000, Cell Signaling), and rabbit anti-β-actin (1:1000, Cell Signaling). .. Washed membranes were incubated in horseradish peroxidase (HRP) conjugated goat anti-mouse or anti-rabbit IgG secondary antibodies (Abcam) diluted in PBS-T supplemented with 10% starting block solution for 45 min at room temperature (RT).

    Article Title: Pulsatility and high shear stress deteriorate barrier phenotype in brain microvascular endothelium
    Article Snippet: Membranes were blocked for 60min in phosphate-buffered saline (PBS, Lonza) containing 0.05% Tween-20 (PBS-T, Thermo Fisher Scientific, Waltham, MA, USA) and 5% nonfat dry milk (LabScientific Inc., Livingston, NJ, USA). .. Then, membranes were incubated overnight in primary antibody at 4 C. Primary antibodies were diluted in PBS-T supplemented with 10% starting block solution (Thermo Fisher Scientific): rabbit anti-Zona Occludens 1 (ZO-1, 1:1000, Life Technologies), mouse antiClaudin-5 (1:2000, Life Technologies), rabbit anti-Pglycoprotein (1:1000, Abcam, Cambridge, MA, USA), rabbit anti-GLUT1 (1:1000, Abcam), rabbit anti-phosphorylated-ERK (1:2000, Cell Signaling, Beverly, MA, USA), rabbit anti-ERK (1:1000, Cell Signaling), rabbit anti-phosphorylated-Src (1:1000, Cell Signaling), rabbit anti-Src (1:1000, Cell Signaling), and rabbit anti-b-actin (1:1000, Cell Signaling). .. Washed membranes were incubated in horseradish peroxidase (HRP) conjugated goat anti-mouse or anti-rabbit IgG secondary antibodies (Abcam) diluted in PBS-T supplemented with 10% starting block solution for 45min at room temperature (RT).

    Article Title: Isthmin-1 (Ism1) modulates renal branching morphogenesis and mesenchyme condensation during early kidney development
    Article Snippet: .. Antibodies used for Western blotting, Co-IP and Integrin signaling blocking include mouse anti-ACTB (1:5000, A5316, Sigma), rabbit anti-GAPDH (1:5000, 10494-1-AP, Proteintech), mouse anti-FLAG (1:2000, F1804, Sigma), rabbit anti-Ism1 (1:5000, Genescript), mouse anti-V5 (1:3000, R960-25, Invitrogen), rabbit anti-phosphorylated FAK (1:1000, #3283, CST), rabbit anti-phosphorylated AKT (1:1000, #9271, CST), rabbit anti-phosphorylated ERK (1:1000, #4370, CST), rabbit anti-phosphorylated SRC (1:1000, #2101, CST), mouse anti-N-cadherin (1:2000, #610920, BD), Goat anti-Gdnf (1:2000, AF212, R&D), HRP-conjugated mouse (1:10000, GE Healthcare, NA9310) and HRP-conjugated rabbit (1:10000, GE Healthcare, NA9340). .. Total RNA was isolated from CMMM-1 cells or tissues by TRIzol reagent (RNAiso Plus, Takara Bio, #9109) according to the manufacturer’s protocol.

    Incubation:

    Article Title: Pulsatility and high shear stress deteriorate barrier phenotype in brain microvascular endothelium
    Article Snippet: Membranes were blocked for 60min in phosphate-buffered saline (PBS, Lonza) containing 0.05% Tween-20 (PBS-T, Thermo Fisher Scientific, Waltham, MA, USA) and 5% nonfat dry milk (LabScientific Inc., Livingston, NJ, USA). .. Then, membranes were incubated overnight in primary antibody at 4 C. Primary antibodies were diluted in PBS-T supplemented with 10% starting block solution (Thermo Fisher Scientific): rabbit anti-Zona Occludens 1 (ZO-1, 1:1000, Life Technologies), mouse antiClaudin-5 (1:2000, Life Technologies), rabbit anti-Pglycoprotein (1:1000, Abcam, Cambridge, MA, USA), rabbit anti-GLUT1 (1:1000, Abcam), rabbit anti-phosphorylated-ERK (1:2000, Cell Signaling, Beverly, MA, USA), rabbit anti-ERK (1:1000, Cell Signaling), rabbit anti-phosphorylated-Src (1:1000, Cell Signaling), rabbit anti-Src (1:1000, Cell Signaling), and rabbit anti-b-actin (1:1000, Cell Signaling). .. Washed membranes were incubated in horseradish peroxidase (HRP) conjugated goat anti-mouse or anti-rabbit IgG secondary antibodies (Abcam) diluted in PBS-T supplemented with 10% starting block solution for 45min at room temperature (RT).



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